Fig. 10. Restoration of Hsp27 in cells containing wt ABCA4: (A) wt-ABCA4 containing cells were transfected with Hsp27 and treated with VX-809. (Plasmids were transfected into ABCA4 containing cell lines using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instruction) The data shows that increasing Hsp27 levels causes wt-ABCA4 levels to increase. The combination of Hsp27 overexpression and addition of VX-809 are synergistic. (B) Hsp27 transfection, with or without VX-809 (20 µM). Protein density was normalized to Ezrin. Data were represented as mean ± SEM (n=3). One way Anova was used for statistics. **P<0.01.